The main objective of this article is to see whether Kitb/kitlgb is necessary for HSCs in the zebrafish model, whether osm is a novel cytokine important for HSCs in the zebrafish model. zebrafish, whether osmr and kitb signaling are sequentially required for HSC specification and whether osmr and kitb function synergistically to expand HSCs in caudal hematopoietic tissue. These are important because understanding the molecular pathways that control hematopoietic stem cell specification and expansion is necessary to develop and implement regenerative drugs. Say no to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get the original essayHa:si:ch73-47f2. 1 is the zebrafish ortholog of mammalian OSM, the gene that encodes the osm protein Ho:si:ch73-47f2. 1 is not the zebrafish ortholog of the mammalian OSM4. As osm expression was not detectable by in situ hybridization (IHS), CECs (caudal embryonic cells) were isolated by FACS (fluorescence-activated cell sorting). This is a method of sorting a mixture of cells one by one into separate containers using electrical charges based on the light scattering and fluorescent properties of each cell. Once the cells were isolated, they were amplified by qPCR (quantitative polymerase chain reaction). This is done by denaturing a target DNA strand, hybridizing it with another strand to isolate the area of ​​interest, and elongating it as in normal transcription. The difference is that a fluorescent label is added to quantify the amplified DNA as it is amplified. The data shown in the figures are averages of biological triplicates, meaning that the entire procedure was performed 3 separate times to control for biological variation. eGFP+ on the x-axis is an enhanced green fluorescent protein used in qPCR to detect the presence of the target. protein, in this case osm, on the zebrafish flk1 gene. On the y-axis, a fold change simply represents the increase in protein expression. A change from 0 to 50 plotted on the y-axis represents a change by a factor of 2. This means that the gene expresses twice as much on 100 as on 50. In the graph, it is evident that there has been more twice as many changes. as much osm expression in the caudal hematopoietic tissue (CHT) or in the tail as in the rest of the embryo. Based on the results on the graph, it is evident that osm is expressed in high volumes in the CHT of model zebrafish. Osm expression in zebrafish, as in mammals, indicates a role in hematopoiesis (blood cell formation), also as in mammals, supporting the hypothesis that si:ch73-47f2. 1 is the ortholog of the mammalian OSM gene which encodes the osm protein involved in hematopoiesis, thus failing to reject the null hypothesis. Keep in mind: this is just a sample. Get a personalized article from our expert writers now. Get a custom article essayThe information shows how osm expands HSCs in caudal hematopoietic tissue. This shows that there was a significant increase in the number of HSCs in the HTCs of embryos injected with osm mRNA. Osmr is the receptor that binds to osm to activate it. This shows that osm and its receptor osmr play a role in the expansion of HSCs in the CHT. One of the main questions of this article was to see if there is an expansion of HSCs (hematopoietic stem cells) in hematopoietic tissue.