Pain is a symptom of illness or damage that occurs most frequently. Pain serves to remind, protect and facilitate the diagnosis of disease. Pain is a protective mechanism of the body that would occur in the event of damage to body tissues1. Current analgesic medications, such as opioids and nonsteroidal anti-inflammatory drugs (NSAIDs), are thought to not be helpful in all cases due to their side effects and low potency2. NSAIDs are associated with gastric irritation, bleeding, ulcers and perforation3. It then becomes necessary to look for other alternatives. The history of medicinal plants is closely linked to the history of civilization. According to the World Health Organization, approximately 80% of the population in many countries still uses medicinal plants for primary health care due to poverty and lack of access to modern medicine4. Since the most important analgesic prototypes (e.g. salicylic acid and morphine) were originally derived from plant sources, the study of plant species traditionally used as analgesics should be a fruitful research strategy in research. new analgesic and anti-inflammatory drugs. to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get the original essay Carica papaya is a medicinal plant native to Central America, which has spread to different parts of the world, Africa and Nigeria. It contains two major bioactive compounds, namely papain and chymopapain, which are used in the brewing, winemaking, textile and tanning industries6. Carica papaya is a medicinal plant known because it contains substances that can be used for therapeutic purposes. This herb has been recommended as anti-ulcerogenic, anti-amoebic, antifungal, antimicrobial, antitumor, hypolipidemic and used in wound healing activity, free radical scavenging activity, diuretic activity, uterotonic activity and antifertility activity7, 8. The Nsukka natives chew the dry seeds of Carica papaya to relieve persistent headaches (migraines), reduce swollen sores and reduce high blood pressure. The methanolic extract of Carica papaya seeds appears to have antinociceptive and anti-inflammatory activity in mice and rats9. Therefore, the present study was undertaken to evaluate the analgesic activity of aqueous extract of Carica papaya seeds in mice. Plant material and extraction: Four ripe and unripe fruits of Carica papaya were collected from a local market and authenticated. The papaya fruits were cut into pieces and the wet seeds were separated. These were then rinsed gently but thoroughly twice in tap water and completely air dried at room temperature for 4 weeks. The dried seeds were pulverized into a fine powder using a household blender-grinder. 50 g of powdered seeds were boiled in 500 ml of distilled water for 30 minutes, after which they were filtered using a piece of clean white cotton gauze. The filtrate was evaporated to complete dryness at 40°C, producing a fine, odorous, chocolate-colored solid residue (yield: 22.5%, w/w). The extraction process was repeated 4 times and the solid residue was weighed and collected in an airtight container and stored in a refrigerator at 4°C. From this fresh preparation, we prepared whenever necessary. Experimental animals: Mice weighing between 25 and 30 g were divided into.