Cell culture and transformation of Arabidopsis T87 cells cultured in suspension of the ecotype Arabidopsis thaliana (L.) Columbia were maintained at 22 °C in JPL3 medium with continuous lighting and shaking at 100 g. Two-week-old cells were sieved through a 500 μm stainless steel mesh and the remaining filtrate was transferred to a flask containing 20 ml of fresh JPL3 medium for subculture. Transformation of T87 cells was carried out by culturing the cells in B5 medium supplemented with 1 μM 1-naphthalene-acetic acid (NAA) and 40 g L-1 sucrose. Cells were cultured for one day at 22°C under continuous lighting and shaking at 120 g. Then, 10 µL of Agrobacterium grown overnight and transformed with the respective vectors was added to the cell suspension and cultured for two additional days. After co-culture, the cell suspension was washed three times with 10 mL of JPL3 medium supplemented with Carbencilin (250 µg mL-1) by centrifugation at 100 g for two minutes. Finally, the cells were resuspended and plated on a JPL3 selection agar plate supplemented with carbenciline (250 µg mL-1), Kanamycin (30 µg mL-1) and...