Use the same TSA sequence plate. Determine whether catalase is produced by the breakdown of hydrogen peroxide. Proceed by adding reactive hydrogen peroxide and watch out for bubbles. In other words, bubbling is caused by the breakdown of hydrogen peroxide and the production of oxygen gas (Benson, 2010). The control for this test is S. aureus. Blood agar plate A streak for isolation is made on the plate. Additionally, check the plaque for hemolysis. Look for the body's ability to produce hemolysin. Bacteria containing hemolysin will generate different patterns. To start, the green part around the colony is alpha. Second, complete clearing around the colony is a good thing. Third, cloudiness or lack of result is gamma. The controls are K. pneumoniae, B. cereus, and S. saprophyticus, in order based on previous results. Simmons Citrate Agar Slant The use of this control is to discover whether or not an organism is capable of using citrate as a carbon source. Place a needle of your body along the surface of the slope. Then start at the bottom and work your way up with the needle. The already publicized indicator is bromothymol blue. An increase in PH will generate a bright blue for a positive result. Control is E.