Nowadays, people around the world are demanding a more sustainable source of energy than other conventional fuels such as coal, natural gas and fossil fuels for their businesses daily. However, conventional fuels are classified as non-renewable energy sources. So, to solve this problem, an alternative fuel called biofuel is used to replace conventional fuels. Additionally, biofuels may attract growing interest in many developing countries by utilizing the "modern" use of biomass to produce clean liquid fuels. The use of biomass as a feedstock for biofuels can provide new employment opportunities for people who remain in this region. The biomass commonly used in biofuels is called algae biofuel or hydrocarbons, in which the oil that does not contain toxic or sulfur substances (Demirbas and Fatih Demirbas, 2011). Biofuels can be produced by either first generation biofuel (FGB), second generation biofuel (SGB), third generation biofuel (TGB) or fourth generation biofuel. FGB mainly comes from food crops for which it has created a problem of food competition....